*********************************
There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
*********************************
Xiangxi Meng
BME PhD Defense Presentation
Date: June 5, 2019
Time: 21:00 EDT
Location: UAW 4235 (GT), Wangkezhen Bldg 907 (PKU)
Committee members:
Qiushi Ren, PhD (Advisor)
May D. Wang, PhD (Co-Advisor)
Weiqiang Chen, PhD
Changhui Li, PhD
Shuming Nie, PhD
Multiplexed Molecular Imaging in the Second Near-Infrared Window
The second near-infrared window (NIR-II window) is a certain range of near-infrared light with superior ability to penetrate biological tissues. In this research, the basic imaging properties of the NIR-II imaging were first explored. To enable this, equipment for conducting NIR-II imaging were constructed and corresponding fluorescent materials were prepared. A new technique to monitor the NIR-II fluorescence during the synthetic process of the fluorescent material was developed and applied.
Liquid phantoms with Intralipid were used as a model system to verify the imaging ability, and the results obtained were verified with a CLARITY experiment. After obtaining such results, new methods to multiplex NIR-II fluorescence imaging and other biomedical procedures were proposed and explored. Taking the advantage of its special penetration ability, NIR-II imaging could be used to investigate the transient process of the first several seconds of the distribution of fluorescent quantum dots administered via tail-vein injection. NIR-II imaging was also used to visualize the intestine structure and bowel movement real-time on nude mice.
The NIR-II imaging was conducted to visualize the process of megavolt X-ray beam radiotherapy. The Cherenkov emission can be observed during megavolt X-ray beam therapies, but most of the energy concentrates in the blue to UV range. With the NIR-II imaging system, the NIR-II part of the Cherenkov emission was visualized. The Cherenkov photon energy was also transferred to the NIR-II range using the NIR-II emission quantum dots. Finally, the Cherenkov emission of the radioisotope Cu-64 was studied.
