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In partial fulfillment of the requirements for the degree of
Doctor of Philosophy in Biology
in the
School of Biological Sciences
Swetha Garimalla
will defend his dissertation
Integrative Immunotranscriptomics of Long-Lived Plasma Cells
Friday, November 2, 2018
2:00 PM
Molecular Sciences and Engineering, 1201A
Thesis Advisor:
Dr. Greg Gibson
School of Biological Sciences
Georgia Institute of Technology
Committee members:
Dr. Soojin Yi
School of Biological Sciences
Georgia Institute of Technology
Dr. Peng Qui
School of Biological Sciences
Georgia Institute of Technology
Dr. Frances Eun-Hyung Lee
Department of Medicine
Emory University
Dr. Rabin Tirouvanziam
Pediatrics Department of Pediatric Infectious Diseases
Emory University
Summary
Long-lived plasma cells are a key component of serological memory encoded by the adaptive immune response. To date, prior studies of these cell types have largely assessed plasma cells as one homogenous population. The Lee Lab at Emory University provided strong evidence for the presence of subpopulations of plasma cells in the bone marrow and peripheral blood. In this work, I analyze six of these populations: pops 2, 3, and 5 in the peripheral blood and pops A, B, and D in the bone marrow sampled from live human donors. Whole-transcriptome analysis of these six flow-sorted plasma cell populations is compared within and across tissue within this study. Further, I present 4 possible models of development of these plasma cells based on the results. Finally, I performed a single-cell RNA sequencing analysis of the long-lived plasma cells (popD) and present evidence for the possibility of subpopulations of this cell type. This work describes many mechanisms of molecular development of long-lived plasma cells, some of which have been experimentally validated by Dr. Doan Nguyen as a result of these analyses, however, further experimental validation is required to validate all findings in this work.
