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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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Kirsten Parratt
BioE Ph.D. Defense Presentation
9:00 AM, Friday, July 20th, 2018
Parker H. Petit Institute for Bioengineering and Bioscience - Suddath Seminar Room 1128
Advisor: Krishnendu Roy, Ph.D. (Georgia Institute of Technology, Emory University)
Committee:
Robert Guldberg, Ph.D. (Georgia Institute of Technology)
Hang Lu, Ph.D. (Georgia Institute of Technology)
Valeria Milam, Ph.D. (Georgia Institute of Technology)
Johnna Temenoff, Ph.D. (Georgia Institute of Technology, Emory University)
3D Material Cytometry (3DMaC): High-throughput, high replicate Screening of Materials using flow cytometry
Biomaterials have become a common feature in everyday life ranging from disposable daily contact lenses to implanted devices engineered to outlast the patient. There is a great deal of unrealized commercial potential for biomaterials systems and ample interest in determining optimal biomaterials for applications such as tissue engineering and detection of biological analytes. Unlike past challenges in polymer selection, candidate biomaterials need to be tested while also accounting for the complexity of living cells and the variability in biological systems. These challenges can be partially addressed by analyzing a large number of biomaterials in a high-throughput manner with high replicate number; however, such methods are lacking.
This thesis shows how flow cytometry can be adapted to the study of biomaterials. Flow cytometry allows for the automated collection of a large number of unique events in a short time period and is already widely used for cell analyses. Here, biomaterial, specifically hydrogel, constructs are fabricated and a combination of shape-, size-, and fluorescence-barcoding (SSF) enables high-throughput, high replicate, highly multiplexed analyses using imaging flow cytometry. This dissertation illustrates how this new method, 3D Material Cytometry (3DMaC), can be applied to tissue engineering and analyte detection, and discusses how the method can be extended to additional biomaterial studies.
