*********************************
There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
*********************************
Johnathan Lyon
BME Ph.D. Defense Presentation
12:00 PM, Friday October 13, 2017
Engineered Biosystems Building (EBB), Children's Healthcare of Atlanta Seminar Room
Georgia Institute of Technology
Advisor: Ravi Bellamkonda, Ph.D. (Duke University)
Committee:
Robert Butera, Ph.D. (Georgia Institute of Technology)
Tobey MacDonald, M.D. (Emory University)
Mark Prausnitz, Ph.D. (Georgia Institute of Technology)
Susan Thomas, Ph.D. (Georgia Institute of Technology)
Investigating Differential Electrotaxis of Glioblastoma and Medulloblastoma Spheroidal Aggregates
Treatment of brain and nervous system cancers remains a daunting clinical challenge, with one of the most common brain malignancies, glioblastoma, incurring a mere 5% five-year survival rate. In the search for new therapeutic means—specifically, new ways to direct or curb the invasion of brain cancers—we investigate the directed invasion of brain cancer cellular aggregates by an electrical field. This property is known as electrotaxis (or galvanotaxis), and is known to be involved in a variety of endogenous phenomena including tissue development and wound healing, and is purported to be involved in cancer invasion and metastasis. In this study, we explore electrotaxis in the context of brain cancer, and provide new insights into the effect’s underlying mechanisms.
Here, we have developed an electrotaxis assay that adapts existing cancer invasion assays and allows us to study electrotaxis on aggregated populations of cells. We characterize glioblastoma and medulloblastoma cell lines in these assays and report on their electrotactic properties. We then investigate the transcriptome for two cell lines that were found to have opposing electrotactic responses, followed by pharmacological inhibition studies to further validate some of our findings. Overall, the hypotheses explored in this work advance our understanding of the pathways that underlie electrotactic sensing and steering and may lead to new means for guiding or managing brain malignancies.
