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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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Shereka Banton
BME PhD Defense Presentation
March 7th, 2017 at 9 am
Manufacturing Related Disciplines Complex (MRDC), Rm 3403
Thesis Committee Members:
Gilda Barabino, PhD (Advisor)
Michael Davis, PhD (Co-advisor)
Wilbur Lam, MD-PhD
Philip Santangelo, PhD
Betty Pace, MD
Abdullah Kutlar, MD
Lakeshia Taite, PhD
Human Peripheral Reticulocyte Isolation and Exosome Release in vitro
The exosomes released by peripheral reticulocytes were originally thought to function as vehicles for protein clearance for the maturing cells. With the emergence of exosomes as mediators of intercellular communication, a new paradigm exists for the role of reticulocyte-derived exosomes in both healthy and disease states, particularly conditions whose pathology is driven by the red blood and its precursors. However, no standard or detailed method for the isolation of human peripheral CD71+ reticulocytes exists. A combination of density-dependent and immunomagnetic approaches was used to demonstrate a procedure to isolate human CD71+ reticulocytes from peripheral blood. Nearly 90% of the CD71+ cells were distinct from the CD71- population when measured with flow cytometry detection of RNA. CD71+ reticulocyte-derived exosomes were then isolated and analyzed after incubation in vitro, the first such demonstration of these phenomena using these cells. These findings form the basis for more targeted and mechanistic studies into the role of reticulocyte-derived exosomes in pathologies like sickle cell disease.
