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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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Hao Xie
PhD proposal presentation
Beijing clock: 9:00 pm May 19th, 2016
location at Beijing: New COE 1st building, meeting room 210
Atlanta clock: 9:00 am May 19th, 2016
location at GT: BME bldg 1103
advisor:
Peng Xi, PhD (Peking University, BME)
co advisor:
Philip J Santangelo, PhD (Georgia Institute of Technology, BME)
committee:
Changhui Li, PhD (PKU, BME)
Tianyu Xie, PhD (PKU, BME)
Juntao Gao, PhD (THU, National Laboratory for Information Science and Technology)
Abstract
Fluorescence microscopy plays an important role in biological researches. Conventional fluorescence microscopy has several limitations: First, resolution of microscope is restricted by the diffraction limit. Second, out of focus light contributes significant background. Third, photobleach deteriorates image quality. Finally, sequential 2D imaging along z axis increases the acquisition time. Recent developed methods like confocal microscopy, light sheet microscopy, light field microscopy, stochastic optical reconstruction microscopy (STORM) are used to overcome some of those problems. The goal of our project is to develop high resolution microscopy systems with faster speed and multi modalities. First we developed Schiliren confocal microscopy, a method which implements phase contrast module to a confocal microscope. A photomultiplier tube (PMT) is used to collect the image instead of charge-coupled device (CCD), so the phase image could be obtained together with the confocal images. Further, we will apply volumetric imaging techniques to those existing microscopy techniques to improve their imaging speed. We will show these techniques provides additional information in cell and tissue researches.
