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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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Advisor:
Jeff Jianzhong Xi, PhD, Department of Biomedical Engineering, Peking University
May Dongmei Wang, PhD, Department of Biomedical Engineering, Georgia Institute of Technology & Emory University
Committee Members:
Huaiqiu Zhu, PhD, Department of Biomedical Engineering, Peking University
Haifeng Chen, PhD, Department of Biomedical Engineering, Peking University
Yangming Wang, PhD, Institute of Molecular Medicine, Peking University
Title: Construction of Cas9-Avd system to Enhance Homologous Recombination Efficiency
Abstract: Homologous recombination is responsible for the maintenance of genome integrity. By introducing an artificial DNA template as donor, certain exogenous sequences could be inserted into genome precisely. But the efficiency is low either in mammlian cell lines or embryos. In my proposal, a novel strategy to enhance homologous recombination efficiency is described and constructed, based on Avidin and Biotin interaction mechanism. This system works well based on primary data and shows huge potential to application.
