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Hao Zhang
PhD Proposal Presentation
Date: December 17, 2015 (Wednesday)
Time: 9:00 pm (Beijing) / 8:00 am (Atlanta)
Location:
New 1st COE Building,Room 208 (PKU campus at Beijing)
U.A. Whitaker Building, Mclnitre Conf. Room (GT campus at Atlanta)
Advisor:
Peng Xi, PhD, Department of Biomedical Engineering, Peking University
Cheng Zhu, PhD, Department of Biomedical Engineering, Georgia Institute of Technology & Emory University
Committee Members:
Tianyu Xie, PhD, Department of Biomedical Engineering, Peking University
Changhui Li, PhD, Department of Biomedical Engineering, Peking University
Juntao Gao, PhD, TNLIST, Tsinghua University
Title: Single Molecular Orientation Detection Microscopy
Abstract: In the past, fluorescence polarization techniques with wide-field, confocal, two-photon excitation (TPE), total internal reflection fluorescence (TIRF) and fluorescence recovery after photobleaching (FRAP) techniques were used to study protein structure and function. All these techniques were obstructed by the same problem: the observation volume is usually a complex system with more than one emitter. To reduce the number of molecules within an observation volume is crucial, as well as to find parameters describing the system instead of a single orientation. Utilizing the tunable transition rates of ON/OFF states of organic dyes or PA-FPs, single molecular orientation detection (SMOD) microsocpy introduces single molecular localization technique into fluorescence polarization imaging. Through imaging both intensity and dipole orientation of fluorescence probes in complicated systems at single molecular accuracy, Single Molecular Orientation Detection (SMOD) microscopy would be a powerful technique in studying structural organization of proteins and lipids.
