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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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Gang Bao, Ph.D. (Advisor)
Michelle Dawson, Ph.D.
Raphael Lee, M.D., Sc.D. (University of Chicago)
Todd Sulchek, Ph.D.
Johnna Temenoff, Ph.D.
Directing the Migration of Mesenchymal Stem Cells and Bystander Cells using Superparamagnetic Iron Oxide Nanoparticles
Cell migration plays an important role in numerous normal and pathological processes. The physical mechanisms of adhesion, protrusion/extension, contractions, and polarization can regulate cell migration speed, persistence time, and downstream effects in paracrine and endocrine signaling. Methods for understanding these biophysical and biochemical responses to date have been limited to the use of external forces acting on mechanotransductive receptors. Additionally, as the use of magnetic nanoparticles for cell tracking and cell manipulation studies continues to gain popularity, so does the importance of understanding the cellular response to mechanical forces caused by these magnetic systems.
This thesis work utilizes superparamagnetic iron oxide nanoparticles and static magnets to induce an endogenous magnetic force on the cell membrane. This cell manipulation model is used to better understand the mechanobiologal responses of mesenchymal stem cell to SPIO labeling and endogenous force generation. Directionally persistent motility, cytoskeletal reorganization, and altered pro-migratory cytokine secretion is reported in this thesis as a response to SPIO based cell manipulation.
