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There is now a CONTENT FREEZE for Mercury while we switch to a new platform. It began on Friday, March 10 at 6pm and will end on Wednesday, March 15 at noon. No new content can be created during this time, but all material in the system as of the beginning of the freeze will be migrated to the new platform, including users and groups. Functionally the new site is identical to the old one. webteam@gatech.edu
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"Next Gen Cell-Sorting for Next Gen Sequence Detection Analysis"
Alice X. Wang
BD Biosciences
San Jose, CA
The seminar will present how the power of flow cytometry can be exploited to isolate, characterize, phenotype and purify single cells from multiple cell populations prior to gene expression profiling. We describe a complete workflow starting with heterogeneous cancer cells that can be isolated at rapid speed by single cell depositions into 96 well plates using the newly developed flow cytometer from BD Biosciences, the BD FACSJazz™. We demonstrate the use of new analytical tools such as index cell sorting that can help track those individual cells during and after sorting. Finally we demonstrate high fidelity of the sorted cells using single cell gene expression profiling on the NanoString nCounter™ platform.
Lunch will be provided 11:30am-12:00pm in the HSRB Cafeteria.
RSVP here
